On this page
Strict control over degradation of individual proteins at the correct time and space in cells is key for dynamic transitions in cell signaling. Protein modification by the modifier ubiquitin is recognized as a fundamental mechanism to target proteins to the main protease complex in the cell: the proteasome. Cell-free and structural studies have provided mechanistic insight into how ubiquitinated proteins are recognized and degraded by the proteasome. However, many principles and regulators of how ubiquitination, proteasome targeting, target extraction from sub-cellular sites, and fate-determinations at the proteasome in the crowded intracellular space are achieved, remain unknown.
Main questions addressed are: 1) How are immune- and cancer-associated proteins targeted to the proteasome, 2) which other cellular factors control their degradation, 3) are these processes deregulated in cancer and immune disease, and 4) how do they mechanistically determine cellular protein fate decisions?
To identify novel cellular regulators of proteasome-dependent degradation, the Versteeg lab utilizes genome-wide genetic screening approaches. There is a strong focus on the discovery of factors controlling the degradation of disease-associated proteins with different conceptual properties, such as ubiquitin-independent, and activation-coupled proteasomal degradation. Following identification of key players in protein stability, we aim to determine the molecular mechanisms by which these regulators control degradation of their targets using cell-biology approaches, and biochemical in vitro reconstitution.
Gijs Versteeg studied Biomedical Sciences, and obtained his PhD in Molecular Virology at the University of Leiden, The Netherlands. He further continued his research on cellular immune signaling responses as a post-doctoral fellow in the Influenza Center of Excellence at Mount Sinai School of Medicine in New York City. Gijs started his independent research group at the Max Perutz Labs in 2013.
Dr. Bohr-Gasse 9
1030 Vienna
Room: 4.009
+43-1-4277-54637
Most cellular proteins are present in the majority of mammals, and are essential for life. Yet, few of them are only found in humans, and often have non-essential functions. We recently discovered that a human-specific –non-conserved– RING protein is essential for cancer cells. Its genetic deletion de-represses a cellular stress response, thereby bringing a stop to cell division.
Unstructured regions with a diverse amino acid composition in proteins are key for initiating proteasomal degradation. Consequently, repetitive sequences are poor degradation-initiation sites. We recently discovered one of the most unstable proteins in human cells with a three-minute half-life. Unexpectedly, its extreme instability is dependent on a large, repetitive protein loop.
Valentina Budroni
PhD Student +43-1-4277-54164
Room: 4.108
Kathrin Hacker
Technician +43-1-4277-74638
Room: 4.108
Irene Schwartz
PhD Student +43-1-4277-54614
Room: 4.108
Alexandra Shulkina
PhD Student +43-1-4277-54614
Room: 4.108
Adrian Söderholm
PhD Student +43-1-4277-54614
Room: 4.108
Gijs Versteeg
Group Leader +43-1-4277-54637
Room: 4.009
Unanchored K48-Linked Polyubiquitin Synthesized by the E3-Ubiquitin Ligase TRIM6 Stimulates the Interferon-IKKε Kinase-Mediated Antiviral Response.
Rajsbaum, Ricardo; Versteeg, Gijs A; Schmid, Sonja; Maestre, Ana M; Belicha-Villanueva, Alan; Martínez-Romero, Carles; Patel, Jenish R; Morrison, Juliet; Pisanelli, Giuseppe; Miorin, Lisa; Laurent-Rolle, Maudry; Moulton, Hong M; Stein, David A; Fernandez-Sesma, Ana; tenOever, Benjamin R; García-Sastre, Adolfo
The E3-ligase TRIM family of proteins regulates signaling pathways triggered by innate immune pattern-recognition receptors.
Versteeg, Gijs A; Rajsbaum, Ricardo; Sánchez-Aparicio, Maria Teresa; Maestre, Ana M; Valdiviezo, Julio; Shi, Mude; Inn, Kyung-Soo; Fernandez-Sesma, Ana; Jung, Jae; García-Sastre, Adolfo
Chemical inhibition of RNA viruses reveals REDD1 as a host defense factor.
Mata, Miguel A; Satterly, Neal; Versteeg, Gijs A; Frantz, Doug; Wei, Shuguang; Williams, Noelle; Schmolke, Mirco; Peña-Llopis, Samuel; Brugarolas, James; Forst, Christian V; White, Michael A; García-Sastre, Adolfo; Roth, Michael G; Fontoura, Beatriz M A
The Group Versteeg is a member of the special Doctoral Program "Signaling Mechanisms in Cellular Homeostasis" reviewed and funded by the Austrian Research Fund FWF.
Project title: "Inhibition of macrophage activation by TRIM47"
The Versteeg group is associated with the Vienna Doctoral School: PhD track Signaling Mechanisms in Cellular Homeastasis.
Project title: "Inhibition of the antiviral immune response by TRIM52"
Identifying and exploiting cell-state dependent metabolic programs
11:00 - 12:00
IMP Lecture HallChromatin as a gatekeeper of chromosome replication
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Mind matters. VBC mental health awareness
13:30 - 14:30 IMP Lecture HallThe multiple facets of Hop1 during meiotic prophase
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Chromosomes as Mechanical Objects: from E.coli to Meiosis to Mammalian cells
11:00 - 12:00 IMBA/GMI Lecture HallConvergent evolution of CO2-fixing liquid-liquid phase separation
11:30 - 12:30 GMI Orange Seminar RoomViral envelope engineering for cell type specific delivery
16:00 - 17:00 IMP Seminar Room 1.014/16New ways of leading: inclusive leadership and revising academic hierarchies
14:15 - 15:15 IMP Lecture HallHow an opportunistic human pathogen colonizes surfaces - From pathogen behavior to new drugs
13:15 - 14:15 UBB - Lecture Hall 1Title to be announced
14:00 - 15:00 Lecture Hall AandB, VBC5Decoding Molecular Plasticity in the Dark Proteome of the Nuclear Pore Complex
11:00 - 12:00 IMBA/GMI Lecture HallProbing the 3D genome architectural basis of neurodevelopment and aging in vivo
17:00 - 18:00 ZoomHow to tango with four - the evolution of meiotic chromosome segregation after genome duplication
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Multidimensional approach to decoding the mysteries of animal development
13:15 - 14:15 UBB - Lecture Hall 1Membrane remodeling proteins at the junction between prokaryotes and eukaryotes
13:15 - 14:15 UBB - Lecture Hall 1Connecting mitotic chromosomes to dynamic microtubules - insight from biochemical reconstitution
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Neurodiversity in academia: strengths and challenges of neurodivergence
14:00 - 15:00 IMP Seminar Room 1.014/16Gene expression dynamics during the awakening of the zygotic genome
11:00 - 12:00 IMBA/GMI Lecture HallWhen all is lost? Measuring historical signals
13:15 - 14:15 UBB - Lecture Hall 1Suckers and segments of the octopus arm
11:00 - 12:00 IMBA/GMI Lecture HallUsing the house mouse radiation to study the rapid evolution of genes and genetic processes
13:15 - 14:15 UBB - Lecture Hall 1CRISPR jumps ahead: mechanistic insights into CRISPR-associated transposons
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Title to be announced
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Enigmatic evolutionary origin and multipotency of the neural crest cells - major drivers of vertebrate evolution
13:15 - 14:15 UBB - Lecture Hall 1Visualising mitotic chromosomes and nuclear dynamics by correlative light and electron microscopy
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Bacterial cell envelope homeostasis at the (post)transcriptional level
13:15 - 14:15 UBB - Lecture Hall 1Polyploidy and rediploidisation in stressful times
13:15 - 14:15 UBB - Lecture Hall 1Prdm9 control of meiotic synapsis of homologs in intersubspecific hybrids
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)RNA virus from museum specimens
13:15 - 14:15 UBB - Lecture Hall 1Programmed DNA double-strand breaks during meiosis: Mechanism and evolution
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Title to be announced
11:00 - 12:00