The endoplasmic reticulum connects to the nucleus by constricted junctions that mature after mitosis.
Bragulat-Teixidor Helena, Ishihara Keisuke, Szücs Gréta Martina, Otsuka Shotaro
Correlative light and electron microscopy at defined cell cycle stages in a controlled environment.
Bragulat-Teixidor Helena, Otsuka Shotaro
Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle.
Deolal Pallavi, Scholz Julia, Ren Kaike, Bragulat-Teixidor Helena, Otsuka Shotaro
Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes.
Forer Arthur, Otsuka Shotaro
A quantitative map of nuclear pore assembly reveals two distinct mechanisms.
Otsuka Shotaro, Tempkin Jeremy O B, Zhang Wanlu, Politi Antonio Z, Rybina Arina, Hossain M Julius, Kueblbeck Moritz, Callegari Andrea, Koch Birgit, Morero Natalia Rosalia, Sali Andrej, Ellenberg Jan
A mitotic chromatin phase transition prevents perforation by microtubules.
Schneider Maximilian W G, Gibson Bryan A, Otsuka Shotaro, Spicer Maximilian F D, Petrovic Mina, Blaukopf Claudia, Langer Christoph C H, Batty Paul, Nagaraju Thejaswi, Doolittle Lynda K, Rosen Michael K, Gerlich Daniel W
Visualizing Nuclear Pore Complex Assembly In Situ in Human Cells at Nanometer Resolution by Correlating Live Imaging with Electron Microscopy.
Bragulat-Teixidor Helena, Hossain M Julius, Otsuka Shotaro
Chemogenetic Control of Nanobodies.
Farrants Helen, Tarnawski Miroslaw, Müller Thorsten G, Otsuka Shotaro, Hiblot Julien, Koch Birgit, Kueblbeck Moritz, Kräusslich Hans-Georg, Ellenberg Jan, Johnsson Kai
Postmitotic nuclear pore assembly proceeds by radial dilation of small membrane openings.
Otsuka Shotaro, Steyer Anna M, Schorb Martin, Hériché Jean-Karim, Hossain M Julius, Sethi Suruchi, Kueblbeck Moritz, Schwab Yannick, Beck Martin, Ellenberg Jan
Mechanisms of nuclear pore complex assembly - two different ways of building one molecular machine.
Otsuka Shotaro, Ellenberg Jan
Nuclear pore assembly proceeds by an inside-out extrusion of the nuclear envelope.
Otsuka Shotaro, Bui Khanh Huy, Schorb Martin, Hossain M Julius, Politi Antonio Z, Koch Birgit, Eltsov Mikhail, Beck Martin, Ellenberg Jan