The Monoclonal Antibody Facility generates mouse monoclonal antibodies against any custom antigen of choice, including short peptides, recombinant proteins, post-translational modifications, or small compounds. Our services comprise immunizations of mice (Balb/c), screening of mouse sera by Western blot and/or ELISA, fusion of splenocytes and screening of fusion clones, as well as the production of the resulting monoclonal antibodies. We put a particular emphasis on the robust and detailed antibody validation in close collaboration with our customers. In contrast to other commercial sources, which offer peptide-ELISA positive clones, we validate every antibody for the recognition of full-length antigens by Western blotting. In addition, we strongly encourage our customers to screen in their own preferred applications for the desired antibodies at the earliest possible stage, i.e. already in the mouse immune sera. Ourfacility was established in 2009. Since then, we have carried out over 130 projects for more than 50 customers, and we have generated a total of about 200 western blot validated monoclonal antibodies.
The Monoclonal Antibody Facility offers in-house development of mouse monoclonal antibodies to researchers at Max Perutz Labs and the Medical University of Vienna but also to academic and non-academic customers outside the Max Perutz Labs. Our aim is to produce novel high quality monoclonal antibodies custom-tailored for the specific needs and applications of our customers.
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Stephan Krämer
Sharon Bone