The facility offers access to state-of-the-art cell and large particle sorting technologies and analysis tools, while also providing dedicated support and adaptable configurations to assist researchers.We are partner of the VLSI Programm (Vienna Life-Science Instruments). https://www.vlsi.at/
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Our work at the facility goes far beyond merely providing access to the machines. We are dedicated to cultivating collaborative relationships and supporting users throughout every step of the experimental process. To this end, we offer researchers expertise in experimental design, sample preparation, staff-operated cell sorting, analyzer assistance, and valuable guidance on data analysis. We are committed to improve quality, and maintain consistency and robustness of work carried out at the facility. In achieve this, the instruments are quality-control checked so that users can rely on the acquired data.
Through our consultation services, we offer comprehensive guidance encompassing various aspects of your research process, including sample preparation, experimental design, instrumentation choices, and data analysis strategies.
We offer personalized support on weekdays from 9:00 AM to 5:00 PM, excluding national holidays. Additionally, each sorting experiment undergoes a post-experiment purity control check to ensure optimal performance and quality.
Our aim is to enable independent access to cell analyzers and sorters, even beyond peak hours. We achieve this through a thorough hands-on training program, ensuring users feel confident. Our staff is available during core hours for immediate advice and guidance.
ZE5
Lasers 5: 355nm, 405nm, 488nm, 561nm, 640nm
Detectors: 27 colors, one side scatter, two forward scatters
Sample input: rack of 40x 5ml tubes, rack of 24x 1.5ml tubes, and 96/384/custom well plates
Temperature control: 4-25°C
Software: Everest
Small particle detector: 405nm FSC
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Calibur
Lasers 2: 488nm, 635nm
Detectors: 4 colors, one side scatter, one forward scatter
Sample input: 5ml tube
Temperature control: no
Software: CellQuest
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Fortessa
Lasers 4: 405nm, 488nm, 561nm, 640nm
Detectors: 16 colors, one side scatter, one forward scatter
Sample input: 5ml tube, and 96/384/custom well plates
Temperature control: no
Software: FACSDiva
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Aurora CS
Lasers 5: 355nm, 405nm, 488nm, 561nm, 640 nm
Detectors: 64 colors, two side scatters, one forward scatter
Sample input: 5ml and 15ml tubes
Sorting output: up to 6-ways bulk, and 96/384 well plates
Temperature control: 4-25°C
Nozzles: 70µm and 100µm
Software: SpectroFlo
Small particle detector: 405nm SSC
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Aria
Lasers 5: 375 nm / 405nm, 488nm, 561nm, 633nm
Detectors: 18 colors, one side scatter, one forward scatter
Sample input: 5ml and 15ml tubes
Sorting output: up to 4-ways bulk, and 96/384 well plates
Temperature control: 4-25°C
Nozzles: 70µm, 85µm, 100µm and 130µm
Software: FACSDiva
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
Melody
Lasers 3: 405nm, 488nm, 561nm
Detectors: 8 colors, one side scatter, one forward scatter
Sample input: 5ml tube
Sorting output: up to 4-ways bulk, and 96/384 well plates
Temperature control: no
Nozzles: 100µm
Software: FACSChorus
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
BioSorter
Lasers 3: 405nm, 488nm, 561nm
Detectors: 6 colors, object length, optical density
Sample input: 50ml tube, 1l tank, and 96/384 well plates
Sorting output: 1-way bulk, and 96/384 well plates
Temperature control: no
FOCA: 250µm, 1000µm
Software: FlowPilot
Large particle detector: 10 - 750µm (Large cells, Cell Clusters, C.elegans, Drosophila, Zebrafish larvae)
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
Internals, please register on PPMS via the Max Perutz Labs intranet link:
Externals, please contact the BioOptics FACS team directly at:
If you are already registered in PPMS, please follow the link below. If you do not have an account, please register first.
Max Perutz Labs Main Building
Dr.-Bohr-Gasse 9, 1030 Vienna
Level 2, Room 2.528 (Aria, Melody, BioSorter)
Level 2, Room 2.530 (ZE5, Calibur, Fortessa)
Autonomous users can book and operate cytometers independently, even outside peak hours. To become an autonomous user, please apply for training through the PPMS system:
Go to the 'Home' dashboard and click on 'Request Training.'
After we receive your training request, we will arrange a hands-on training session and confirm it via email.
If you do not have a PPMS account, please register first.
Do NOT forget to bring your own sample for the training session!
As the evaluation of our facility relies in part on proper acknowledgment in publications, we kindly request that you recognize our contributions in the following ways:
For routine analysis, please mention the facility as the "Max Perutz Labs BioOptics FACS Facility" in the acknowledgment section of your manuscript.
For significant intellectual and/or experimental contributions from the facility staff, please acknowledge them as co-authors.
To analyze your data, you may borrow our FlowJo Dongle located in room 2.530, by the window. Please note that it is intended for local use only, so kindly refrain from taking it off-site. If you wish to use it on your own computer, complete the form (include your name, date, and room number) and return it promptly.
Internal users have the option to request access to the FlowJo Portal Group License. For inquiries, please contact the facility directly at facs@maxperutzlabs.ac.at.
Kitti Dora Csalyi
Head of Facility 
+43 1 4277 24051
Room: 2.530
Endre Kiss
Facility Staff Scientist +43 1 4277 61782
Room: 2.530
Thomas Sauer
Facility Staff Scientist +43 1 4277 61782
Room: 2.530
Martin Leeb
Group Leader +43 1 4277 74644
Room: 3.123
Gijs Versteeg
Group Leader +43 1 4277 54637
Room: 4.009