The facility offers access to state-of-the-art cell and large particle sorting technologies and analysis tools, while also providing dedicated support and adaptable configurations to assist researchers.We are partner of the VLSI Programm (Vienna Life-Science Instruments). https://www.vlsi.at/
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Our work at the facility goes far beyond merely providing access to the machines. We are dedicated to cultivating collaborative relationships and supporting users throughout every step of the experimental process. To this end, we offer researchers expertise in experimental design, sample preparation, staff-operated cell sorting, analyzer assistance, and valuable guidance on data analysis. We are committed to improve quality, and maintain consistency and robustness of work carried out at the facility. In achieve this, the instruments are quality-control checked so that users can rely on the acquired data.
Through our consultation services, we offer comprehensive guidance encompassing various aspects of your research process, including sample preparation, experimental design, instrumentation choices, and data analysis strategies.
We offer personalized support on weekdays from 9:00 AM to 5:00 PM, excluding national holidays. Additionally, each sorting experiment undergoes a post-experiment purity control check to ensure optimal performance and quality.
Our aim is to enable independent access to cell analyzers and sorters, even beyond peak hours. We achieve this through a thorough hands-on training program, ensuring users feel confident. Our staff is available during core hours for immediate advice and guidance.
ZE5
Lasers 5: 355nm, 405nm, 488nm, 561nm, 640nm
Detectors: 27 colors, one side scatter, two forward scatters
Sample input: rack of 40x 5ml tubes, rack of 24x 1.5ml tubes, and 96/384/custom well plates
Temperature control: 4-25°C
Software: Everest
Small particle detector: 405nm FSC
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Calibur
Lasers 2: 488nm, 635nm
Detectors: 4 colors, one side scatter, one forward scatter
Sample input: 5ml tube
Temperature control: no
Software: CellQuest
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Fortessa
Lasers 4: 405nm, 488nm, 561nm, 640nm
Detectors: 16 colors, one side scatter, one forward scatter
Sample input: 5ml tube, and 96/384/custom well plates
Temperature control: no
Software: FACSDiva
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Aurora CS
Lasers 5: 355nm, 405nm, 488nm, 561nm, 640 nm
Detectors: 64 colors, two side scatters, one forward scatter
Sample input: 5ml and 15ml tubes
Sorting output: up to 6-ways bulk, and 96/384 well plates
Temperature control: 4-25°C
Nozzles: 70µm and 100µm
Software: SpectroFlo
Small particle detector: 405nm SSC
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.530
Aria
Lasers 5: 375 nm / 405nm, 488nm, 561nm, 633nm
Detectors: 18 colors, one side scatter, one forward scatter
Sample input: 5ml and 15ml tubes
Sorting output: up to 4-ways bulk, and 96/384 well plates
Temperature control: 4-25°C
Nozzles: 70µm, 85µm, 100µm and 130µm
Software: FACSDiva
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
Melody
Lasers 3: 405nm, 488nm, 561nm
Detectors: 8 colors, one side scatter, one forward scatter
Sample input: 5ml tube
Sorting output: up to 4-ways bulk, and 96/384 well plates
Temperature control: no
Nozzles: 100µm
Software: FACSChorus
Small particle detector: no
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
BioSorter
Lasers 3: 405nm, 488nm, 561nm
Detectors: 6 colors, object length, optical density
Sample input: 50ml tube, 1l tank, and 96/384 well plates
Sorting output: 1-way bulk, and 96/384 well plates
Temperature control: no
FOCA: 250µm, 1000µm
Software: FlowPilot
Large particle detector: 10 - 750µm (Large cells, Cell Clusters, C.elegans, Drosophila, Zebrafish larvae)
Location: Main Building (Dr.-Bohr-Gasse 9) - Level 2, Room 2.528
Internals, please register on PPMS via the Max Perutz Labs intranet link:
Externals, please contact the BioOptics FACS team directly at:
If you are already registered in PPMS, please follow the link below. If you do not have an account, please register first.
Max Perutz Labs Main Building
Dr.-Bohr-Gasse 9, 1030 Vienna
Level 2, Room 2.528 (Aria, Melody, BioSorter)
Level 2, Room 2.530 (ZE5, Calibur, Fortessa)
Autonomous users can book and operate cytometers independently, even outside peak hours. To become an autonomous user, please apply for training through the PPMS system:
Go to the 'Home' dashboard and click on 'Request Training.'
After we receive your training request, we will arrange a hands-on training session and confirm it via email.
If you do not have a PPMS account, please register first.
Do NOT forget to bring your own sample for the training session!
As the evaluation of our facility relies in part on proper acknowledgment in publications, we kindly request that you recognize our contributions in the following ways:
For routine analysis, please mention the facility as the "Max Perutz Labs BioOptics FACS Facility" in the acknowledgment section of your manuscript.
For significant intellectual and/or experimental contributions from the facility staff, please acknowledge them as co-authors.
To analyze your data, you may borrow our FlowJo Dongle located in room 2.530, by the window. Please note that it is intended for local use only, so kindly refrain from taking it off-site. If you wish to use it on your own computer, complete the form (include your name, date, and room number) and return it promptly.
Internal users have the option to request access to the FlowJo Portal Group License. For inquiries, please contact the facility directly at facs@maxperutzlabs.ac.at.
Kitti Dora Csalyi
Facility Head 
+43-1-4277-24051
Room: 2.530
Thomas Sauer
Facility Manager +43-1-4277-61782
Room: 2.530
Martin Leeb
Group Leader +43-1-4277-74644
Room: 3.123/3.125
Gijs Versteeg
Group Leader +43-1-4277-54637
Room: 4.009
Nutrient-regulated control of lysosome function by signaling lipid conversion
14:00 - 15:00
VBC5 Lecture Hall A+B/ZoomShedding Light on the Dark Side of Terrestrial Ecosystems: Assessing Biogeochemical Processes in Soils
12:00 - 13:00 ZoomProtein homeostasis and lifelong cell maintenance
11:00 - 12:00 IMBA/GMI Lecture HallDissecting the turgor sensing mechanisms in the blast fungus Magnaporthe oryzae
11:00 - 12:00 GMI Orange Seminar RoomPikobodies: What does it take to bioengineer NLR immune receptor-nanobody fusions
14:00 - 15:00 GMI Orange Seminar roomWhen all is lost? Measuring historical signals
13:15 - 14:15 UBB - Lecture Hall 1Gene regulatory mechanisms governing human development, evolution and variation
13:00 - 14:00 IMBA/GMI Lecture HallRegulation of Cerebral Cortex Morphogenesis by Migrating Cells
11:00 - 12:00 IMBA/GMI Lecture HallPhage therapy for treating bacterial infections: a double-edged sword
13:30 - 14:30 UBB - Lecture Hall 3Suckers and segments of the octopus arm
11:00 - 12:00 IMBA/GMI Lecture HallUsing the house mouse radiation to study the rapid evolution of genes and genetic processes
13:15 - 14:15 UBB - Lecture Hall 1CRISPR jumps ahead: mechanistic insights into CRISPR-associated transposons
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)SLiMs and SHelMs: Decoding how short linear and helical motifs direct PPP specificity to direct signaling
14:00 - 15:00 MFPL main building, 6th floor SR1/2Title to be announced
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Visualising mitotic chromosomes and nuclear dynamics by correlative light and electron microscopy
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Enigmatic evolutionary origin and multipotency of the neural crest cells - major drivers of vertebrate evolution
13:15 - 14:15 UBB - Lecture Hall 1Engineered nanocarriers for imaging of small proteins by CryoEM
11:00 - 12:00 GMI Orange Seminar RoomBacterial cell envelope homeostasis at the (post)transcriptional level
13:15 - 14:15 UBB - Lecture Hall 1Title to be announced
14:00 - 15:00 VBC5 Lecture Hall A+B/ZoomHydrologic extremes alter mechanisms and pathways of carbon export from mountainous floodplain soils
12:00 - 13:00 UBB - Lecture Hall 1Dissecting post-transcriptional gene expression regulation in humans and viruses
11:00 - 12:00 IMBA/GMI Lecture HallPolyploidy and rediploidisation in stressful times
13:15 - 14:15 UBB - Lecture Hall 1Prdm9 control of meiotic synapsis of homologs in intersubspecific hybrids
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Title to be announced
14:00 - 15:00 VBC5 Lecture Hall A+B/ZoomRNA virus from museum specimens
13:15 - 14:15 UBB - Lecture Hall 1Programmed DNA double-strand breaks during meiosis: Mechanism and evolution
11:30 - 12:30 Max Perutz Labs SR 1 (6th floor)Title to be announced
11:00 - 12:00